8TG7
Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.313, 69.222, 70.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.405 - 1.775 |
Rwork | 0.182 |
R-free | 0.23410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.472 |
Data reduction software | autoPROC (1.1.7) |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP (11..4.03) |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.410 | 1.891 |
High resolution limit [Å] | 1.775 | 1.775 |
Rmerge | 0.055 | 1.399 |
Rmeas | 0.060 | 1.516 |
Rpim | 0.024 | 0.576 |
Number of reflections | 16243 | 812 |
<I/σ(I)> | 15.1 | 1.3 |
Completeness [%] | 86.5 | 25.5 |
Redundancy | 6.3 | 6.8 |
CC(1/2) | 0.999 | 0.659 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 2.0 M ammonium sulfate, 0.1 M Tris pH 8.6 |