8TG6
tRNA 2'-phosphotransferase (Tpt1) from Aeropyrum pernix in complex with sulfate anions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | H 3 2 |
Unit cell lengths | 101.679, 101.679, 101.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 66.410 - 1.600 |
R-factor | 0.1449 |
Rwork | 0.143 |
R-free | 0.18180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wfx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.299 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.410 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.063 | 0.623 |
Rpim | 0.016 | 0.156 |
Number of reflections | 26676 | 1302 |
<I/σ(I)> | 31.8 | 4.9 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 17 | 17 |
CC(1/2) | 1.000 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M HEPES-Na, pH 7.5, 1.0 M ammonium sulfate, 2% PEG-400 |