8TFU
Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.810, 68.252, 70.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.149 - 1.482 |
Rwork | 0.181 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.584 |
Data reduction software | autoPROC (1.1.7) |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP (11.4.03) |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.444 | 1.590 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.062 | 1.217 |
Rmeas | 1.330 | |
Rpim | 0.526 | |
Number of reflections | 25018 | 1251 |
<I/σ(I)> | 14.1 | 1.2 |
Completeness [%] | 80.1 | 21.2 |
Redundancy | 6.2 | 6.1 |
CC(1/2) | 0.409 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.48 M sodium phosphate monobasic monohydrate, 1.2 M potassium phosphate dibasic |