8TFP

Fab from C10-S66K antibody in complex with carfentanil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	71.050, 73.492, 95.089
Unit cell angles	90.00, 90.38, 90.00

Refinement procedure

Resolution	34.720 - 1.780
R-factor	0.1789
Rwork	0.178
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.891
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.090	1.810
High resolution limit [Å]	1.780	1.780
Number of reflections	91341	4542
<I/σ(I)>	6.5
Completeness [%]	97.8
Redundancy	3.2
CC(1/2)	0.965	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1.0 M Li-chloride, 10% PEG-6000, 0.1 M HEPES pH 7.0