8TFE

Crystal structure of Fab fragment of anti-HCV E2 antibody (CBH-7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	79.935, 101.912, 109.936
Unit cell angles	90.00, 100.24, 90.00

Refinement procedure

Resolution	46.140 - 1.620
R-factor	0.17881
Rwork	0.177
R-free	0.21384
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6x9x
RMSD bond length	0.011
RMSD bond angle	1.649
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.090	0.544
Rmeas	0.098	0.595
Rpim	0.038	0.238
Number of reflections	107383	5314
<I/σ(I)>	27.75
Completeness [%]	98.4	98.5
Redundancy	6.4	5.6
CC(1/2)	0.981	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Sodium phosphate dibasic pH 4.2, 0.2 M NaCl, and 20% PEG8000