8TEM
Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in the inward-facing conformation in lauryl maltose neopentyl glycol (LMNG)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | OTHER |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.02 |
| Software | RELION,Leginon,PHENIX |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
