8TEC

Crystal structure of Kindlin2 in complex with acylated beta1 integrin peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	73.487, 102.093, 76.029
Unit cell angles	90.00, 96.81, 90.00

Refinement procedure

Resolution	75.490 - 2.040
R-factor	0.1955
Rwork	0.194
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xpy
RMSD bond length	0.007
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.090	2.090
High resolution limit [Å]	2.040	2.040
Rmerge	0.069	1.728
Rmeas	0.075
Rpim	0.028
Number of reflections	69860	3942
<I/σ(I)>	12.8	0.9
Completeness [%]	98.8	87.4
Redundancy	6.8	6.1
CC(1/2)	0.999	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris, pH 8.5, 10% isopropanal