8TE2

16mer self-complementary duplex RNA with D:C pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2023-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.038413
Spacegroup name	H 3 2
Unit cell lengths	41.562, 41.562, 125.383
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.220 - 1.630
R-factor	0.2143
Rwork	0.210
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.098
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.790	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.093	0.362
Rmeas	0.105	0.424
Rpim	0.048	0.216
Number of reflections	5477	285
<I/σ(I)>	10	2.4
Completeness [%]	99.7	97.1
Redundancy	8.3	6.9
CC(1/2)	0.998	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	400 mM Sodium chloride, 120 mM Calcium chloride, 27% v/v (+/-)-2-Methyl-2,4-pentanediol, 20 mM MES pH 5.8