8TE2
16mer self-complementary duplex RNA with D:C pair sequence 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2023-04-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.038413 |
Spacegroup name | H 3 2 |
Unit cell lengths | 41.562, 41.562, 125.383 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.220 - 1.630 |
R-factor | 0.2143 |
Rwork | 0.210 |
R-free | 0.25160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 2.098 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.790 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.093 | 0.362 |
Rmeas | 0.105 | 0.424 |
Rpim | 0.048 | 0.216 |
Number of reflections | 5477 | 285 |
<I/σ(I)> | 10 | 2.4 |
Completeness [%] | 99.7 | 97.1 |
Redundancy | 8.3 | 6.9 |
CC(1/2) | 0.998 | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 400 mM Sodium chloride, 120 mM Calcium chloride, 27% v/v (+/-)-2-Methyl-2,4-pentanediol, 20 mM MES pH 5.8 |