8TDY
16mer self-complementary duplex RNA with D:U pair sequence 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2023-03-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.038413 |
Spacegroup name | H 3 2 |
Unit cell lengths | 41.146, 41.146, 125.047 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.680 - 1.660 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.22380 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.097 | 0.520 |
Rmeas | 0.104 | 0.569 |
Rpim | 0.035 | 0.225 |
Number of reflections | 5058 | 483 |
<I/σ(I)> | 4.2 | 4.2 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 8.5 | 6.1 |
CC(1/2) | 1.000 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 80 mM Strontium chloride hexahydrate, 20 mM Magnesium chloride hexahydrate, 40 mM Sodium cacodylate trihydrate pH 7.0, 20% v/v (+/-)-2-Methyl-2,4-pentanediol, 12 mM Spermine |