8TDR

Crystal structure of the methyltransferase domain of DNMT3A homotetramer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 3
Unit cell lengths	179.429, 179.429, 108.065
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.290 - 3.320
R-factor	0.2108
Rwork	0.210
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.440
High resolution limit [Å]	3.320	7.150	3.320
Rmerge	0.220	0.042	1.319
Rmeas	0.254	0.049	1.538
Rpim	0.127	0.024	0.782
Total number of observations	225061
Number of reflections	57297	5722	5689
<I/σ(I)>	3.3
Completeness [%]	100.0	99.8	100
Redundancy	3.9	4.1	3.7
CC(1/2)		0.998	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2-0.4 M potassium sodium tartrate