8TCR
Structure of glucose bound Alistipes sp. 3-Keto-beta-glucopyranoside-1,2-Lyase AL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.1808 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.517, 113.679, 208.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.080 |
| R-factor | 0.18429 |
| Rwork | 0.182 |
| R-free | 0.22898 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.694 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.130 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.060 | 0.530 |
| Rmeas | 0.085 | 0.749 |
| Rpim | 0.060 | 0.530 |
| Total number of observations | 130468 | 8537 |
| Number of reflections | 68867 | 4460 |
| <I/σ(I)> | 8.9 | 1.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.997 | 0.566 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG 1500 0.13 M MIB pH 5 |
