8TCH

Initiation of replication protein with helicase activity encoded by Pathogenicity Island SaPIBov1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979415
Spacegroup name	H 3
Unit cell lengths	115.408, 115.408, 310.687
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.572 - 2.700
R-factor	0.2659
Rwork	0.265
R-free	0.28150
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge		0.105	1.058
Rmeas	0.157	0.126	1.351
Rpim	0.069	0.069	0.830
Number of reflections	42298	2110	2107
<I/σ(I)>	4.2
Completeness [%]	99.7	99.8	99.2
Redundancy	2.5	2.6	2.5
CC(1/2)		0.966	0.284

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	288.15	0.1M MES, pH6,-25% Jeffermine M-600-12.5% Ethylene Glycol-5mM DTT