8TCE
Lipoprotein(a) Kringle IV domain 8 - Lp(a) KIV8 in complex with LY3353871
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.602, 57.574, 38.305 |
| Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
| Resolution | 18.740 - 1.070 |
| R-factor | 0.16214 |
| Rwork | 0.161 |
| R-free | 0.18291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.740 | 1.130 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.068 | 0.607 |
| Number of reflections | 61826 | 5622 |
| <I/σ(I)> | 7.9 | 1.8 |
| Completeness [%] | 91.1 | 91.1 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1000 mM trisodium citrate pH 7.0, 100 mM HEPES pH 6.0 |
