8TCA

Structure of a mouse IgG antibody antigen-binding fragment (Fab) targeting N6-methyladenosine (m6A), an RNA modification, no ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	79.906, 79.906, 145.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.000 - 2.020
R-factor	0.1835
Rwork	0.181
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.183
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.092
High resolution limit [Å]	2.020	2.020
Rmerge	0.218	2.290
Rmeas	0.234	2.466
Rpim	0.082	0.888
Number of reflections	31198	2999
<I/σ(I)>	9.71	1.08
Completeness [%]	98.2	96.71
Redundancy	7.4	7.3
CC(1/2)	0.996	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 2K [20% (v/v)], MgCl2 [0.2 M], Tris pH 8.0 [0.1 M], glycerol [5-15% (v/v)]