8TC9

Human asparaginyl-tRNA synthetase bound to OSM-S-106

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	115.088, 126.810, 161.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.460 - 2.000
R-factor	0.1835
Rwork	0.182
R-free	0.22070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xix
RMSD bond length	0.007
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.460	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.180	2.306
Rmeas	0.212	2.718
Rpim	0.111	1.419
Number of reflections	159099	7783
<I/σ(I)>	6.4	0.8
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.997	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% (v/v) glycerol, 40 mM potassium phosphate and 14% polyethylene glycol 8,000, and 100-mM Tris pH 7.6