8TC8
Human asparaginyl-tRNA synthetase bound to adenosine 5'-sulfamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 115.851, 126.285, 160.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.290 - 1.900 |
R-factor | 0.1827 |
Rwork | 0.181 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xix |
RMSD bond length | 0.007 |
RMSD bond angle | 0.860 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.290 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.163 | 2.601 |
Rmeas | 0.192 | 3.070 |
Rpim | 0.072 | 1.186 |
Number of reflections | 185028 | 9037 |
<I/σ(I)> | 7.5 | |
Completeness [%] | 99.9 | |
Redundancy | 6.9 | |
CC(1/2) | 0.998 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% (v/v) glycerol, 40 mM potassium phosphate and 14% polyethylene glycol 8,000, and 100-mM Tris pH 7.6 |