8TC7

Human asparaginyl-tRNA synthetase, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	113.490, 127.195, 163.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.580 - 1.900
R-factor	0.1823
Rwork	0.180
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.172
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.100	2.021
Rmeas	0.117	2.386
Rpim	0.061	1.253
Number of reflections	185343	8986
<I/σ(I)>	9.5	0.8
Completeness [%]	99.8
Redundancy	6.9
CC(1/2)	0.999	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% (v/v) glycerol, 40 mM potassium phosphate and 14% polyethylene glycol 8,000, and 100-mM Tris pH 7.6