8TBX

Crystal structure of human DDX1 helicase in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	50.708, 71.517, 138.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.800 - 2.710
R-factor	0.20678
Rwork	0.203
R-free	0.28647
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.374
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.151	0.094	0.967
Rmeas	0.163	0.103	1.046
Rpim	0.061	0.040	0.392
Total number of observations	100389
Number of reflections	14191	804	691
<I/σ(I)>	5
Completeness [%]	99.9	100	100
Redundancy	7.1	6.8	6.9
CC(1/2)	0.995	0.987	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.04M Potassium phosphate monobasic,16%w/v PEG 8K,20% v/v Glycerol