8TBJ

Tricomplex of RMC-7977, KRAS G12A, and CypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95375
Spacegroup name	P 21 21 21
Unit cell lengths	65.450, 81.460, 127.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.610 - 1.450
R-factor	0.1868
Rwork	0.185
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.523
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.610	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.104	1.363
Rmeas	0.112	1.463
Rpim	0.041	0.529
Number of reflections	120928	11961
<I/σ(I)>	8.48	1.18
Completeness [%]	99.9	99.85
Redundancy	7.3	7.6
CC(1/2)	0.998	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	24% PEG4000, 0.1 M Tris-HCl, pH 8.0