8TBF
Tricomplex of RMC-7977, KRAS WT, and CypA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.95375 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.757, 84.240, 125.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.290 - 1.500 |
R-factor | 0.1794 |
Rwork | 0.178 |
R-free | 0.21040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.939 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.290 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.059 | 0.389 |
Rmeas | 0.063 | 0.418 |
Rpim | 0.023 | 0.151 |
Number of reflections | 112271 | 11048 |
<I/σ(I)> | 17.1 | 4.54 |
Completeness [%] | 99.8 | 99.57 |
Redundancy | 7.3 | 7.4 |
CC(1/2) | 0.997 | 0.932 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 24% PEG4000, 0.1 M Tris-HCl, pH 8.0 |