8TB2

Structure of SasG (type II) (residues 165-421) from Staphylococcus aureus MW2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793384
Spacegroup name	P 21 21 2
Unit cell lengths	78.600, 70.210, 43.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.360 - 1.880
Rwork	0.184
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.360	1.947
High resolution limit [Å]	1.880	1.880
Rmerge	0.067	0.297
Rmeas	0.094
Number of reflections	20226	1980
<I/σ(I)>	5.81	2.25
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.991	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	100 mM HEPES (pH 7.8), 200 mM Ammonium Sulfate, 25%-33% BCS PEG SMEAR Medium