8T9U

Human PU.1 ETS-domain (165-270) in complex with d(AATAAGCGIAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999990
Spacegroup name	P 1 21 1
Unit cell lengths	43.021, 60.622, 44.562
Unit cell angles	90.00, 116.79, 90.00

Refinement procedure

Resolution	33.260 - 1.470
R-factor	0.1469
Rwork	0.145
R-free	0.18060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.260	1.523
High resolution limit [Å]	1.470	1.470
Rmerge	0.063	0.344
Rmeas	0.070	0.386
Rpim	0.031	0.172
Number of reflections	34426	3415
<I/σ(I)>	14.56	3.37
Completeness [%]	98.6	98.13
Redundancy	5.2	4.9
CC(1/2)	0.996	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350