8T9N
Bacillus subtilis RsgI GGG mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-04-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.175, 63.889, 59.935 |
| Unit cell angles | 90.00, 92.66, 90.00 |
Refinement procedure
| Resolution | 38.130 - 1.900 |
| R-factor | 0.2343 |
| Rwork | 0.230 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.688 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.130 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.028 | 0.655 |
| Number of reflections | 22424 | 1431 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM HEPES-KOH pH 7.5, 100 mM ammonium acetate, 28.5% PEG-3350 |
