8T8S
Sortilin-PGRN peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999982608809 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 237.030, 237.030, 89.427 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 205.270 - 2.990 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.453 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 205.270 | 3.240 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.093 | 0.450 |
| Number of reflections | 58113 | 12291 |
| <I/σ(I)> | 10.87 | |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.10 M Tris, 30.00 %w/v PEG 3000, 0.20 M NaCl |
