8T8R
Sortilin-PGRN peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99993 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.945, 78.618, 111.200 |
| Unit cell angles | 90.00, 126.80, 90.00 |
Refinement procedure
| Resolution | 44.560 - 2.870 |
| R-factor | 0.25877 |
| Rwork | 0.258 |
| R-free | 0.28670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.292 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.040 | 3.120 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmerge | 0.034 | 0.430 |
| Number of reflections | 25644 | 5633 |
| <I/σ(I)> | 19.36 | |
| Completeness [%] | 98.9 | 98.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20.00 %w/ PEG 3350, 0.20 M Sodium citrate |
