8T8L

Structure of Domain of Unknown Function 507 (DUF507) in Space Group P3(2)21

Refinement Statistics

Experimental method: SOLUTION SCATTERING, X-RAY DIFFRACTION (1.9 Å)

Cell axes	83.096	83.096	68.796
Cell angles	90.00	90.00	120.00
Spacegroup	P 32 2 1
Resolution limits	41.55 - 1.90

Crystallization Conditions

crystal ID	method	pH	pH range	temperature	unit
1	VAPOR DIFFUSION, SITTING DROP			286

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2022-04-29
Detector	RDI CMOS_8M
Wavelength(s)	1.0722
Spacegroup name	P 32 2 1
Unit cell lengths	83.096, 83.096, 68.796
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.550 - 1.900
R-factor	0.2062
Rwork	0.205
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.634
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.550	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.079	1.611
Rmeas	0.084	1.741
Rpim	0.026	0.643
Total number of observations	221069	9359
Number of reflections	21999	1365
<I/σ(I)>	18.6	1.1
Completeness [%]	99.8
Redundancy	10	6.9
CC(1/2)	0.999	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		286	0.1 M MES monohydrate pH 6.5 and 12% w/v PEG 20,000