8T8B
Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site formyl-MAI-tripeptidyl-tRNA analog ACCA-IAMf at 2.65A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 207.110, 441.130, 612.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 119.320 - 2.650 |
R-factor | 0.2129 |
Rwork | 0.211 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rqb |
RMSD bond length | 0.004 |
RMSD bond angle | 0.845 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 119.320 | 119.320 | 2.720 |
High resolution limit [Å] | 2.650 | 11.850 | 2.650 |
Rmerge | 0.190 | 0.047 | 1.939 |
Rmeas | 0.207 | 0.051 | 2.138 |
Total number of observations | 9752234 | ||
Number of reflections | 1585401 | 18710 | 116896 |
<I/σ(I)> | 8.02 | 30.16 | 1.02 |
Completeness [%] | 99.0 | 98.7 | 99.1 |
Redundancy | 6.151 | 6.797 | 5.725 |
CC(1/2) | 0.995 | 0.997 | 0.169 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |