8T7Z
Crystal structure of alpha-glucosidase (yicI) from Klebsiella aerogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-20 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.788, 208.509, 280.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.250 - 2.700 |
R-factor | 0.2308 |
Rwork | 0.229 |
R-free | 0.26290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.615 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21rc1_4933: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.550 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.185 | 1.169 |
Rmeas | 0.200 | 1.275 |
Rpim | 0.076 | 0.506 |
Total number of observations | 915751 | 40706 |
Number of reflections | 132661 | 6487 |
<I/σ(I)> | 9.4 | 1.8 |
Completeness [%] | 100.0 | |
Redundancy | 6.9 | 6.3 |
CC(1/2) | 0.994 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 291 | JCSG+ A7: 20% (w/v) PEG 8000, 100 mM Ches pH 9.5. KlaeA.19615.a.B1.PW39183 at 20.7 mg/mL. plate 13206 well A7 drop 2 . Puck: PSL-0415, Cryo: 20% PEG 200 + 80% crystallant |