8T7J

Oxygen- and PLP-dependent Cap15 holoenzyme bound with phosphate anion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	142.590, 220.986, 96.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.900 - 2.400
R-factor	0.2257
Rwork	0.224
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	1.501
Rmeas	0.093	1.562
Rpim	0.025	0.462
Number of reflections	60031	4126
<I/σ(I)>	19.3	1.8
Completeness [%]	99.5	99.38
Redundancy	13.5	13
CC(1/2)	1.000	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M sodium acetate, 17 % polyethylene glycol 3350, 10-15 mg/mL Cap15