8T74

Crystal structure of KRAS4a (GMPPNP) in complex with RAF1 (RBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	53.371, 37.703, 70.876
Unit cell angles	90.00, 101.67, 90.00

Refinement procedure

Resolution	46.520 - 1.650
R-factor	0.1739
Rwork	0.173
R-free	0.19580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.709
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.520	46.520	1.750
High resolution limit [Å]	1.650	4.930	1.650
Rmerge	0.043	0.020	0.564
Rmeas	0.051	0.024	0.725
Number of reflections	31826	1310	4128
<I/σ(I)>	15.4
Completeness [%]	94.6
Redundancy	3.3
CC(1/2)	0.999	0.999	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M DL-malic acid (pH 7.0), 20% PEG 3350