8T74
Crystal structure of KRAS4a (GMPPNP) in complex with RAF1 (RBD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.371, 37.703, 70.876 |
Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
Resolution | 46.520 - 1.650 |
R-factor | 0.1739 |
Rwork | 0.173 |
R-free | 0.19580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.709 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.520 | 46.520 | 1.750 |
High resolution limit [Å] | 1.650 | 4.930 | 1.650 |
Rmerge | 0.043 | 0.020 | 0.564 |
Rmeas | 0.051 | 0.024 | 0.725 |
Number of reflections | 31826 | 1310 | 4128 |
<I/σ(I)> | 15.4 | ||
Completeness [%] | 94.6 | ||
Redundancy | 3.3 | ||
CC(1/2) | 0.999 | 0.999 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M DL-malic acid (pH 7.0), 20% PEG 3350 |