8T73
Crystal structure of KRAS4a-R151G with bound GDP and Mg ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 3 |
Unit cell lengths | 84.779, 84.779, 41.735 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.390 - 1.500 |
R-factor | 0.1555 |
Rwork | 0.154 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.142 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.390 | 42.390 | 1.590 |
High resolution limit [Å] | 1.500 | 4.470 | 1.500 |
Rmerge | 0.062 | 0.043 | 0.463 |
Rmeas | 0.067 | 0.047 | 0.518 |
Number of reflections | 52617 | 2030 | 7326 |
<I/σ(I)> | 17.94 | ||
Completeness [%] | 97.3 | ||
Redundancy | 6.5 | ||
CC(1/2) | 0.999 | 0.998 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES pH 7.4, 2 M ammonium sulfate , 0.1 M MgCl2.6H2O |