8T6E
Crystal structure of T33-28.3: Deep-learning sequence design of co-assembling tetrahedron protein nanoparticles
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 1 |
Unit cell lengths | 113.737, 114.050, 114.197 |
Unit cell angles | 62.09, 77.86, 89.41 |
Refinement procedure
Resolution | 48.470 - 2.480 |
R-factor | 0.2052 |
Rwork | 0.205 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.446 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.470 | 2.520 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.062 | 0.630 |
Number of reflections | 171459 | 8425 |
<I/σ(I)> | 6.3 | 0.7 |
Completeness [%] | 98.1 | 97.5 |
Redundancy | 2.4 | 2.2 |
CC(1/2) | 0.994 | 0.341 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.26 M sodium phosphate, 0.14 M potassium phosphate |