8T6C

Crystal structure of T33-18.2: Deep-learning sequence design of co-assembling tetrahedron protein nanoparticles

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2022-05-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	H 3
Unit cell lengths	138.812, 138.812, 129.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.870 - 1.920
R-factor	0.2
Rwork	0.198
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.618
Data reduction software	DIALS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.870	1.960
High resolution limit [Å]	1.920	1.920
Number of reflections	70870	2619
<I/σ(I)>	9.9	0.1
Completeness [%]	100.0	100
Redundancy	6.7	6.7
CC(1/2)	0.997	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.9 M NPS, 0.1 M Tris-Bicine, pH 8.5, 30% v/v glycerol + PEG4000