8T5J
Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Francisella philomiragia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-08 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 61.850, 61.850, 86.611 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.100 - 1.550 |
R-factor | 0.1723 |
Rwork | 0.170 |
R-free | 0.20760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.962 |
Data reduction software | SAINT |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21rc1_4933: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.170 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.097 | 2.648 |
Rmeas | 0.099 | 2.730 |
Rpim | 0.020 | 0.662 |
Total number of observations | 718556 | 23447 |
Number of reflections | 28420 | 1382 |
<I/σ(I)> | 24.7 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 25.3 | 17 |
CC(1/2) | 1.000 | 0.516 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Berkeley D10: 20% (w/v) PEG 3350, 100 mM Hepes pH 7.5, 100 mM Lithium sulfate, 100 mM Magnesium chloride, 10% (w/v) 1,6-Hexanediol. FrphA.20731.a.VL2.PB00125 at 14.5 mg/mL. plate 13468 well D10 drop1, Cryo: 80% crystallant+20% PEG200 |