8T36

Crystal structure of K49 acetylated LC3A in complex with the LIR of TP53INP2/DOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97750
Spacegroup name	P 21 21 21
Unit cell lengths	33.198, 59.939, 62.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.202 - 1.852
R-factor	0.2146
Rwork	0.210
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.237
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.202	1.919
High resolution limit [Å]	1.852	1.852
Number of reflections	10037	728
<I/σ(I)>	8.47
Completeness [%]	90.6
Redundancy	4.6
CC(1/2)	0.995	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG3350, 0.1M Na-citrate, 5% isopropanol, 0.1 M HEPES pH 7.5