8T33

Crystal structure of K46 acetylated GABARAP in complex with the LIR of TP53INP2/DOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.522
Spacegroup name	C 2 2 21
Unit cell lengths	53.488, 89.178, 63.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.870 - 1.599
R-factor	0.1874
Rwork	0.185
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.870	1.657
High resolution limit [Å]	1.599	1.599
Number of reflections	39016	3866
<I/σ(I)>	23.94
Completeness [%]	99.8
Redundancy	4.4
CC(1/2)	0.999	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10 % w/v PEG 3000, 0.2 M Zinc acetate dihydrate, 0.1 M Sodium acetate PH 4.5