8T2P
5TU-t1 - heterodimeric triplet polymerase ribozyme
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5 |
| Software | cryoSPARC,Rosetta,ISOLDE,UCSF ChimeraX,Coot,PHENIX,Amber,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
