8T2O

Crystal structure of SCV PTE G18U RNA in complex with Fab BL3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	77.769, 81.551, 322.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.990 - 3.290
R-factor	0.1825
Rwork	0.176
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.232
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	161.380	3.550
High resolution limit [Å]	3.290	3.290
Rmerge	0.049	1.853
Rmeas	0.053	1.998
Rpim	0.021	0.741
Number of reflections	15976	3266
<I/σ(I)>	18.5	1
Completeness [%]	99.4	99.8
Redundancy	6.8	7.1
CC(1/2)	1.000	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	0.2 M lithium sulfate monohydrate, 0.1 M tris pH 8.6, 20% w/v PEG 3350