8T1W

Crystal structure of orphan G protein-coupled receptor 6 with bound CVN424

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	123
Detector technology	PIXEL
Collection date	2020-11-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	63.731, 63.731, 250.932
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.070 - 3.490
R-factor	0.2929
Rwork	0.291
R-free	0.32190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.634
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.490	3.500
Rmerge	0.060	1.890
Number of reflections	7983	406
<I/σ(I)>	28.4
Completeness [%]	98.0
Redundancy	5.7
CC(1/2)	1.000	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	NaCH3COO, PEG 400, NaCl, PPG P40