8T1T

Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA with QSY deletion) heteromeric complex (bound to SAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-11
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	52.500, 59.300, 60.100
Unit cell angles	95.90, 96.50, 112.60

Refinement procedure

Resolution	46.130 - 1.550
R-factor	0.17493
Rwork	0.173
R-free	0.20866
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.739
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.130	1.650
High resolution limit [Å]	1.550	1.550
Rmeas	0.054	0.715
Number of reflections	87728	15117
<I/σ(I)>	12.53
Completeness [%]	92.1
Redundancy	2.8
CC(1/2)	0.998	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	240 mM sodium malonate pH 5-5.5, 8-12% PEG 3350 at a 1-1 or 2-1 ratio of protein to mother liquor. Protein concentration is 20mg/mL