8T1T
Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA with QSY deletion) heteromeric complex (bound to SAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-11 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.500, 59.300, 60.100 |
| Unit cell angles | 95.90, 96.50, 112.60 |
Refinement procedure
| Resolution | 46.130 - 1.550 |
| R-factor | 0.17493 |
| Rwork | 0.173 |
| R-free | 0.20866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.739 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.130 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmeas | 0.054 | 0.715 |
| Number of reflections | 87728 | 15117 |
| <I/σ(I)> | 12.53 | |
| Completeness [%] | 92.1 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.998 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 240 mM sodium malonate pH 5-5.5, 8-12% PEG 3350 at a 1-1 or 2-1 ratio of protein to mother liquor. Protein concentration is 20mg/mL |
