8T1R
Crystal structure of human CPSF73 catalytic segment in complex with compound 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.803, 82.311, 102.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.250 - 2.200 |
R-factor | 0.1986 |
Rwork | 0.196 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.518 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.250 | 2.330 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 25871 | 4087 |
<I/σ(I)> | 10.2 | 2 |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 5.6 | 5.6 |
CC(1/2) | 0.997 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18-30% (w/v) PEG3350, 200-250 mM ammonium sulfate and 0.1 M Bis-Tris pH 6.3-6.7 |