8T1N

Micro-ED Structure of a Novel Domain of Unknown Function Solved with AlphaFold

Experimental procedure

Collection date	2019-11-01
Spacegroup name	P 21 21 21
Unit cell lengths	40.580, 94.990, 101.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	3.000 - 3.000
R-factor	0.2845
Rwork	0.283
R-free	0.30660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8t0b
RMSD bond length	0.014
RMSD bond angle	1.592

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			3.100
High resolution limit [Å]	3.020	13.490	3.020
Rmerge	0.365	0.244	0.444
Rmeas	0.386	0.281	0.501
Total number of observations	39900
Number of reflections	4846	56	268
<I/σ(I)>	5.58	6.85	2.51
Completeness [%]	58.8
CC(1/2)	0.912	0.808	0.140

Crystallization Conditions

crystal ID	method	pH	temperature	details
1