8T0F

Crystal structure of the PEG10 promoter-bound ONECUT2 DNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-11-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	96.229, 78.303, 39.447
Unit cell angles	90.00, 110.99, 90.00

Refinement procedure

Resolution	29.510 - 2.610
R-factor	0.2306
Rwork	0.228
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.603
Data reduction software	HKL-2000 (v721.3)
Data scaling software	HKL-2000 (v721.3)
Phasing software	MOLREP (11.7.03; 13.07.2020)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.121	0.039	1.292
Rmeas	0.130	0.042	1.389
Rpim	0.048	0.016	0.507
Number of reflections	8330	435	433
<I/σ(I)>	12.7
Completeness [%]	99.1	98.9	98.4
Redundancy	7.3	7.2	7.3
CC(1/2)	1.000	0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.04 M KH2PO4, 16 % PEG 8000 and 20 % Glycerol