8T0A

Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain with a Y270E mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 21 2 21
Unit cell lengths	66.775, 68.644, 110.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.150 - 2.140
R-factor	0.1856
Rwork	0.183
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.815
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.150	2.219
High resolution limit [Å]	2.140	2.142
Rmerge	0.033	0.223
Number of reflections	28425	2656
<I/σ(I)>	14.75	2.95
Completeness [%]	99.3
Redundancy	2
CC(1/2)	0.998	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	PEG4000 15%, NaCl 0.1M, DTT 2mM