8SZS

Cat DHX9 bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	86.110, 86.110, 347.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.030 - 2.380
Rwork	0.218
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	2.450
High resolution limit [Å]	2.380	2.380
Rmerge	0.144	1.542
Rmeas	0.147	1.575
Rpim	0.029	0.317
Total number of observations	1403168	110689
Number of reflections	53722	4579
<I/σ(I)>	17.4	2.4
Completeness [%]	100.0
Redundancy	26.1	24.2
CC(1/2)	0.999	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	291	0.15 M ammonium sulfate, 0.1 M Tris, pH 8.2, 15% w/v PEG4000; 5 mM GDP and 50 mM magnesium chloride in protein solution