8SZR
Dog DHX9 bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95364 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.290, 85.290, 352.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.350 - 2.970 |
| Rwork | 0.243 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.814 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 3.150 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.066 | 1.301 |
| Rmeas | 0.067 | 1.327 |
| Rpim | 0.013 | 0.259 |
| Total number of observations | 734995 | 113359 |
| Number of reflections | 27977 | 4412 |
| <I/σ(I)> | 33 | 3.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 26.3 | 25.7 |
| CC(1/2) | 1.000 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 200 mM Lithium sulfate, 100 mM MES pH 6, 20% (w/v) PEG 4000; 5 mM ADP and 50 mM magnesium chloride in protein solution |
