8SZP

Human DHX9 bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97624
Spacegroup name	C 2 2 21
Unit cell lengths	119.100, 122.260, 349.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.621 - 2.620
Rwork	0.219
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.877
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.130	2.670
High resolution limit [Å]	2.620	2.620
Rmerge	0.062	1.398
Rmeas	0.064	1.450
Rpim	0.018	0.382
Total number of observations	980269	63231
Number of reflections	76541	4516
<I/σ(I)>	21.8	2.2
Completeness [%]	99.7
Redundancy	12.8	14
CC(1/2)	1.000	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	200 mM Lithium sulfate, 100 mM MES pH 6, 20% (w/v) PEG 4000; 5 mM ADP and 50 mM magnesium chloride in protein solution