8SZ5

[2T5] Self-assembling DNA motif with 5 base pairs between junctions and P32 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 32
Unit cell lengths	69.089, 69.089, 59.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.540 - 2.890
R-factor	0.2015
Rwork	0.198
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6x8c
RMSD bond length	0.004
RMSD bond angle	0.583
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.833	59.833	3.169
High resolution limit [Å]	2.890	9.450	2.892
Number of reflections	3687	205	203
<I/σ(I)>	8
Completeness [%]	81.5
Redundancy	11	9.7	9.5
CC(1/2)	0.997	0.997	0.085

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate