8SZ3
Structure of human beta 1,3-N-acetylglucosaminyltransferase 2 with compound 7j
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.375, 76.405, 128.019 |
| Unit cell angles | 90.00, 102.58, 90.00 |
Refinement procedure
| Resolution | 29.140 - 2.320 |
| R-factor | 0.2627 |
| Rwork | 0.261 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jhk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.727 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.140 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.064 | 0.462 |
| Rmeas | 0.076 | 0.557 |
| Rpim | 0.041 | 0.309 |
| Number of reflections | 33310 | 3035 |
| <I/σ(I)> | 11.9 | 2.5 |
| Completeness [%] | 98.4 | 92.6 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M Hepes pH 7.0, 0.2 M Sodium Acetate, 17% PEG 4000, 15% glycerol |
