8SYK
Crystal structure of RNA device 43 truncation mutant 3 (U100C), holo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.223, 81.223, 528.831 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.320 - 3.060 |
| R-factor | 0.2266 |
| Rwork | 0.225 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.480 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 528.840 | 3.210 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.090 | 2.148 |
| Rmeas | 0.095 | 2.239 |
| Rpim | 0.029 | 0.625 |
| Total number of observations | 427293 | 58097 |
| Number of reflections | 35158 | 4553 |
| <I/σ(I)> | 10.8 | 1.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.2 | 12.8 |
| CC(1/2) | 0.997 | 0.464 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.05 M HEPES pH 7.5, 0.01 M MgCl2, 2.5 mM Spermine, 14% MPD |
